LOCUS       40924_4759        5963 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pARO191 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Okamoto S.
  TITLE     cloning vector pARO191 sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Contact:Sho Okamoto National Institute of 
            Genetics, Microbial Genetics Laboratory, Genetics Strains Research 
            Center; 1111 Yata, Mishima, Shizuoka 411-8540, Japan URL 
            :https://www.nig.ac.jp/labs/MicroGen/
REFERENCE   3  (bases 1 to 5963)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5963)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2016) Contact:Sho Okamoto National Institute of Genetics, 
            Microbial Genetics Laboratory, Genetics Strains Research Center; 
            1111 Yata, Mishima, Shizuoka 411-8540, Japan URL 
            :https://www.nig.ac.jp/labs/MicroGen/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     925..941
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    942..998
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1011..1027)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1035..1051)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1059..1089)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1104..1125)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      1413..2001
                     /label=pMB1 ori
                     /note="pMB1 ori"
     misc_feature    2427..2567
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2753..3341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4377..5189)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            complement(5443..5552)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     terminator      5604..5647
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt
       61 cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc
      121 catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg
      181 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta
      241 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc
      301 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg
      361 gaaattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
      421 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      481 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
      541 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
      601 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
      661 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
      721 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
      781 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      841 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      901 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc
      961 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg
     1021 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1081 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1141 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1201 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1261 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1321 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1381 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1441 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1501 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1561 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1621 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1681 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1741 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1801 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1861 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1921 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1981 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2041 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2101 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2161 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2221 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2281 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcacacat gcagctcccg
     2341 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     2401 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     2461 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     2521 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     2581 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2641 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2701 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2761 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2821 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2881 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2941 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3001 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3061 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3121 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3181 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3241 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3301 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3361 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3421 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3481 aaagtatata tgagtaaact tggtctgaca ggtcgcttgc ggtattcgga atcttgcacg
     3541 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca
     3601 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag
     3661 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt
     3721 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc
     3781 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg
     3841 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg
     3901 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag
     3961 ccggcggcac ctcgctaacg gattcaccgt ttttatcagg ctctgggagg cagaataaat
     4021 gatcatatcg tcaattatta cctccacggg gagagcctga gcaaactggc ctcaggcatt
     4081 tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac
     4141 ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa
     4201 tttctgccat tcatccgctt attatcactt attcaggcgt agcaccaggc gtttaagggc
     4261 accaataact gccttaaaaa aaaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4321 tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa
     4381 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     4441 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     4501 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     4561 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     4621 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     4681 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     4741 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     4801 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     4861 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     4921 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     4981 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     5041 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     5101 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
     5161 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     5221 gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5281 agaccccgta gaaaagatca aaggatctat gataagctgt caaacatgag aattgacgcg
     5341 tcaattcgag ggggatcaat tccgtgatag gtgggctgcc cttcctggtt ggcttggttt
     5401 catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag cctngcagag
     5461 caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc
     5521 tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata caccaagtct
     5581 agataagttc aggatgaatt ccagaaatca tccttagcga aagctaagga ttttttttat
     5641 ctgaaattct gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5701 tcttagacgt caggtggcac gggatctcga cgctctccct tatgcgactc ctgcattagg
     5761 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
     5821 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     5881 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
     5941 taggcgccag caaccgcacc tgt
//
